restriction site meaning in English
限制位置
Examples
- In this case , two restriction sites ( ecor i , hind iii ) were added to the primers ( p1 , p2 ) separately
扩增产物经双酶切后，重组到表达载体pet - 32a ( + )中。 - Oligomap - oligomap is a tool to help researchers engineer restriction sites into oligonucleotides using silent mutagenesis and reverse translation algorithms
Oligomap是一个帮助研究人员使用哑诱变和反向转换算法来约束寡核苷酸位置点的工具。 - 2 . ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220 . construction of non - fusion expression plasmid pbv220 - endostatin
用ecori和bamhi酶切endostatincdna的pcr产物，将其插入到质粒载体pbv220中相应的限制性酶切位点，构建非融合质粒表达载体pbv220 - endostatin 。 - A pair of primers containing sph i and hind iii restriction sites were designed , according to the poifn - a gene in ddbj / genbank . then poifn - a gene was cloned from porcine genomic dna by pcr
根据ddbj genbank基因库中已登录的猪干扰素基因序列，设计了含sph和hind酶切位点的一对引物，采取聚合酶链式反应( pcr )法，以猪基因组dna为模板进行了poifn克隆。 - Ba - dfe mature peptide - coding sequence was cloned to the restriction site ncol and ndei of the expression vector pet - 15b , respectively , and highly expressed in e . coli bl21 ( de3 ) by the form of non - fusion and his - badfe fusion protein
Lmmol liptg诱导时，融合表达时产生分子量约为30kd 、带有his标签的ba dfe融合蛋白，非融合表达时则产生分子量为28kd的ba dfe 。